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DEFINITION
The Wondfo® Barbiturates Assay (BAR) is a flow-through lateral immunochromatographic assay for the detection of barbiturates in human urine at a threshold concentration of 300 ng/ml or higher.
BRIEF DESCRIPTION
Barbiturates are a class of drugs that inhibit the central nervous system. They have a wide range of half-lives, from 2 to 40 hours, and may be detected in the urine within 1 to 4 days after use. Phenobarbital is a long-acting barbiturate derivative, used as a daytime sedative and very often as an anticonvulsant. Pentobarbital and secobarbital are examples of sedatives from the group of short-acting barbiturates.
Abuse of barbiturates can lead not only to impaired coordination and mental disturbances, but also to respiratory arrest, coma, and even death. Barbiturates are administered orally, rectally, or by intravenous and intramuscular injections. Short-acting barbiturates are usually excreted in the urine as metabolites, while long-acting barbiturates are excreted mostly unchanged.
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PRECAUTIONS
This test is for external use only. Do not swallow.
2. Dispose of after the first use. The test must not be used more than once.
3 Do not use the test kit after the expiration date.
4. Do not use the kit if the package is damaged or poorly sealed.
5. Keep out of the reach of children.
6. Do not evaluate results later than 10 minutes.
ASSAY KIT
One package contains the test and a moisture absorber.
The moisture absorber is for storage only and is not used in the analysis.
STORAGE AND STABILITY
Store at 2 ° C - 30 ° C in a closed container until expiration date.
Store in a place protected from direct sunlight, moisture and high temperature.
Do not freeze.
SAMPLE COLLECTION AND PREPARATION
Collect urine sample into a urine cup (container). Urine samples can be stored in the refrigerator (2 °C - 8 °C) for up to 48 hours. For longer storage, samples should be frozen (-20°C or lower).
Frozen or refrigerated samples must be brought to room temperature before testing. Use only pure aliquots for the test.
TEST PROCEDURE
The test should be performed at room temperature (10 °C to 30 °C)
1. Remove the test strip from the sealed package.
2. Immerse the strip in the urine so that the arrow points in the direction of the urine. Remove the strip after at least 10 seconds and place it horizontally on a clean, dry, non-absorbent surface (such as the neck of a urine container).
3. Evaluate the results after 5 minutes.
DO NOT interpret the test results later than 10 minutes.
IMPORTANT: Make sure that the urine level does not go beyond the MAX line (marking line), otherwise the test will not be performed correctly.
INTERPRETATION OF RESULTS
Positive (+)
A pale pink band is visualized in the test section. A colored band does not appear in the test section. A positive result indicates that the barbiturate concentration is at or above the detection limit (300 ng/ml).
Negative (-)
A pale pink band is visualized in the control area and in the test area. A negative result indicates that the barbiturate concentration is zero or below the limit of determination (300 ng/ml).
Invalid
If the stained strip is not visualized in the control area or if the stained strip is only visualized in the test area, the test results are invalid.
LIMITATIONS OF THE PROCEDURE
1. This test was developed for testing urine samples only. The efficacy of this test when using other samples has not been validated.
2. If tainted urine samples are used, erroneous results may be obtained. Strongly oxidizing agents such as bleach (hypochlorite) can oxidize drug samples. If a tampered sample is suspected, obtain a new sample.
3. This test is a qualitative screening test. It is not intended to quantify drug concentrations or intoxication levels.