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DEFINITION
The Wondfo® Amphetamine Detection Test (AMP) is a flow-through lateral chromatographic immunoassay to detect amphetamine in human urine at a threshold concentration of 1000 ng/ml or higher.
BRIEF DESCRIPTION
Amphetamines are immediately absorbed in the gastrointestinal tract and then deactivated by the liver or excreted unchanged in the urine with a half-life of approximately 12:00. These substances can be detected in the urine 1-2 days after use. Amphetamines are metabolized to disaminated (hippuric acid and benzoic acid) and hydroxylated metabolites. Methamphetamine is partially metabolized to amphetamine and its main active metabolite. Amphetamines increase heart rate and blood pressure and suppress appetite. Some studies have noted that heavy abuse can cause permanent damage to major nerve structures in the brain.
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PRECAUTIONS
This test is for external use only. Do not swallow.
2. Dispose of after the first use. The test must not be used more than once.
3 Do not use the test kit after the expiration date.
4. Do not use the kit if the package is damaged or poorly sealed.
5. Keep out of the reach of children.
6. Do not evaluate results later than 10 minutes.
ASSAY KIT
One package contains the test and a moisture absorber.
The moisture absorber is for storage only and is not used in the analysis.
STORAGE AND STABILITY
Store at 2 ° C - 30 ° C in a closed container until expiration date.
Store in a place protected from direct sunlight, moisture and high temperature.
Do not freeze.
SAMPLE COLLECTION AND PREPARATION
Collect urine sample into a urine cup (container). Urine samples can be stored in the refrigerator (2 °C - 8 °C) for up to 48 hours. For longer storage, samples should be frozen (-20°C or lower).
Frozen or refrigerated samples must be brought to room temperature before testing. Use only pure aliquots for the test.
TEST PROCEDURE
The test should be performed at room temperature (10 °C to 30 °C)
1. Remove the test strip from the sealed package.
2. Immerse the strip in the urine so that the arrow points in the direction of the urine. Remove the strip after at least 10 seconds and place it horizontally on a clean, dry, non-absorbent surface (such as the neck of a urine container).
3. Evaluate the results after 5 minutes.
DO NOT interpret the test results later than 10 minutes.
IMPORTANT: Make sure that the urine level does not go beyond the MAX line (marking line), otherwise the test will not be performed correctly.
INTERPRETATION OF RESULTS
Positive (+)
A pale pink band is visualized in each test region. A colored band does not appear in the corresponding test region. A positive result indicates that the amphetamine concentration is at or above the detection limit (1000 ng/ml).
Negative (-)
A pale pink band is visualized in the control and test areas. A negative result indicates that the amphetamine concentration is zero or below the limit of determination (1000 ng/mL).
Invalid
If the stained strip is not visualized in the control area or if the stained strip is only visualized in the test area, the test results are invalid.
LIMITATIONS OF THE PROCEDURE
1. This test was developed for testing urine samples only. The efficacy of this test when using other samples has not been validated.
2. If tainted urine samples are used, erroneous results may be obtained. Strongly oxidizing agents such as bleach (hypochlorite) can oxidize drug samples. If a tampered sample is suspected, obtain a new sample.
3. This test is a qualitative screening test. It is not intended to quantify drug concentrations or intoxication levels.